Table 2.

Species-specific reference chemical clearance (CL_int) and Heat-Treated Control (HTC) recovery

Species / Reference chemical (CAS No.)	CLint (µL/min/106 hepatocytes)a	Concentration (µM)b	n c	HTC recovery % ± SD	n d	Source
Human / Verapamil (52–53-9)	24.1 (11.1–45.8)	0.1	10	101 ± 10	7	This study
	16.4 (7.37–28.8)	1	10	100 ± 9	7
	21.8 (4.65–49.0)	0.25–3	29	-	-	Published literaturee
Rat / Fenbuconazole (114369–43-6)	26.9 (10.6–45.5)	0.1	9	102 ± 12	7	This study
	17.6 (6.79–37.6)	1	9	112 ± 43	7
	3.89	1	1	-	-	Wetmore et al., 2013
Trout / Pyrene (129–00-0)	27.9 (12.7–46.9)	0.1	7	115 ± 41	6	This study
	7.33 (2.32–13.2)	1	7	103 ± 34	6
	45.7 (19.3–66.7)	0.025–0.25	3	-	-	Published literaturee

^aValues shown for the present study represent means (low–high) for n runs for each starting chemical concentration measured using two lots of human and rat, and four lots of trout hepatocytes. Values given for published literature are reported as means (low–high) for n references which utilized a starting chemical concentration within 5-fold of those employed in the present study.

^bValues shown for the present study represent starting chemical concentrations. Values given for published literature represent the range of reported starting chemical concentrations (low–high).

^cSample sizes are given as the number of CL_int values determined in the present study (Table S9) or the number of values obtained from published literature

^dSample sizes are given for the number of HTC recovery values determined for each species and starting chemical concentration

^eReferences for published literature on verapamil and pyrene are given in Table S10