Table 2.

NBS data from 35 retrospective specimens from confirmed CAH cases in New Zealand in the period 2005–2017. All 17OHP and 21DF concentrations are nmol/L whole blood. For the purposes of the study, a positive screen was second-tier 17OHP or 21DF above laboratory cut-offs.

									LCMSMS Data
Case No.	Year	Sex	Age	GA	BW (g)	1st Tier 17OHP	2nd Tier 17OHP Immuno	Protocol 1 Screening Result	17OHP	21DF	Protocol 2 Screening Result
12	2017	M	3	37	3010	278	140	Positive	95	5	Positive
13	2014	M	3	41	4330	401	326	Positive	232	6	Positive
14	2014	F	3	41	3750	319	93	Positive	46	3	Positive
15	2013	F	3	38	3610	209	179	Positive	114	2	Positive
16	2013	F	1	36	2580	91	38	Positive	20	19	Positive
17	2013	F	1	41	4200	242	211	Positive	212	209	Positive
18	2012	F	2	40	3650	211	160	Positive	61	14	Positive
19	2012	M	4	40	3165	69	53	Positive	36	10	Positive
20	2012	F	1	40	4100	108	56	Positive	44	11	Positive
21	2011	M	3	39	2970	207	142	Positive	84	10	Positive
22	2010	F	1	34	2410	295	239	Positive	158	34	Positive
23	2010	F	0	38	-	144	111	Positive	76	24	Positive
24	2010	F	1	39	3710	305	131	Positive	73	44	Positive
25	2009	M	2	31	1810	354	305	Positive	194	38	Positive
26	2009	F	5	40	3200	301	201	Positive	268	47	Positive
27	2008	M	4	40	3750	135	66	Positive	42	2	Positive
28	2008	M	3	-	4340	282	63	Positive	39	19	Positive
29	2008	M	2	39	3350	255	186	Positive	97	<2	Positive
30	2008	F	2	41	3605	326	267	Positive	115	66	Positive
31	2007	F	3	-	4360	248	215	Positive	86	38	Positive
32	2007	M	2	41	4330	415	185	Positive	81	21	Positive
33	2006	M	3	40	3620	404	575	Positive	326	9	Positive
34	2006	F	3	42	3230	123	66	Positive	33	48	Positive
35	2005	F	2	39	3340	444	285	Positive	160	12	Positive
36	2005	F	4	-	3760	127	67	Positive	41	16	Positive
CAH Cases Not Detected by Screening
37	2014	M	4	-	4770	30	17	Negative	30	5	Positive
38	2010	F	4	38	2930	72	19	Negative	19	13	Positive
39	2010	M	2	-	3655	34	17	Negative	8	7	Positive
40	2009	F	3	40	3360	30	21	Negative	12	11	Positive
41	2015	F	2	39	3450	5	-	Negative	2	<2	Negative
42	2015	M	3	39	3770	26	-	Negative	12	10	Negative *
43	2012	F	4	39	3640	13	-	Negative	6	<2	Negative
44	2010	F	3	40	3110	6	-	Negative	1	<2	Negative
45	2008	F	5	31	1640	4	-	Negative	2	<2	Negative
46	2006	F	3	40	3530	11		Negative	4	<2	Negative
Laboratory Screening Cut-offs						≥27	≥23		≥27	≥3

* Case 42 is a negative screening result using protocol 2 as the specimen would not reflex to second-tier testing.