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. 2022 Oct 23;17:464. doi: 10.1186/s13018-022-03284-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — PCED1B-AS1 siRNA silencing downregulated miR-10a via methylation. PCED1B-AS1 was downregulated and miR-10a was upregulated in U2OS and MG-63 (A). The role of PCED1B-AS1 siRNA in miR-10a accumulation and the role of miR-10a in PCED1B-AS1 accumulation (B) were studied with RT-qPCR. The role of PCED1B-AS1 siRNA silencing in miR-10a gene methylation was studied with MSP (C). *p < 0.05