Figure 5.

CDR3 in HHC2 and μ cDNA. Top, HHC2 junctions (accession numbers MN888965-MN888974). Bottom, Junctions in μ cDNA. Each group was compared to known HHC2 and μ GL flanks, of which representative VH, D, and JH flanks are shown at the top, as labeled. The first codon is TGT (codon 92, Kabat numbering) and the last codon, in JH, is GGN (codon 104), part of the JH Gly-X-Gly motif. A space has been inserted in front of codon 103, almost always TGG in vertebrate HCDR3, in order to show where the end of CDR3 is regarded in both HHC2 and μ in this study. Highlighted clone names indicate nonproductive VDJ. Encoded Arg and Lys are indicated in red. Contribution to CDR3 from D and N/P additions are shown in the column at right. The average number of nucleotides (± S.D.) in D/N/P was calculated without the asterisked values from non-productive sequences.