Figure 8.

Distribution of amino acids in H chain CDR3. Amino acid frequency was tallied for CDR3 from efish μ, HHC1, HHC2, and nurse shark μ (all five subfamilies), and two G2 subfamily H chains from two spleen cDNA libraries (unpublished data). Sequence information was used from IgNAR [73] and from llama VHH1 and VHH3 subfamilies, chosen for the absence of disulfide bonding between CDR [74]. The CDR3 sequences according to Kabat numbering [36] include positions 95–102 or IMGT positions 107–117.