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MS parameters of components.
| Components | tR (min) | Ion mode | MRM | Q1 pre bias (V) | Collision energy (V) | Q3 pre bias (V) |
|---|---|---|---|---|---|---|
| Gallic acid | 1.736 | ESI(–) | 169.10 → 125.05 | 18 | 17 | 20 |
| Salidroside | 2.183 | ESI(–) | 345.20 → 299.15 | 23 | 11 | 29 |
| Chlorogenic acid | 2.89 | ESI(–) | 353.20 → 191.05 | 24 | 18 | 30 |
| Puerarin | 3.367 | ESI(–) | 415.20 → 267.00 | 28 | 33 | 25 |
| Ethyl gallate | 5.962 | ESI(–) | 197.10 → 124.00 | 21 | 23 | 21 |
| Ferulic acid | 6.498 | ESI(–) | 193.10 → 134.00 | 21 | 14 | 25 |
| Tetrahydropalmatine | 7.037 | ESI(+) | 355.60 → 192.10 | –25 | –24 | –14 |
