Fig. 2.

Quantification of down-regulated (0.45 ± 0.06) intact N-glycopeptide YHNQTLR_N2H5F0S0 from cation-independent mannose-6-phosphate receptor (P11717, MPRI_HUMAN) on N-glycosite N400 in MCF-7/ADR relative to MCF-7 cells. a–c the isotopic envelope fingerprinting maps of the precursor ions from three technical replicates; d selective fragmentation and the graphical fragmentation map of N-glycan moiety with the peptide backbone, e fragmentation and the graphical fragmentation map of the peptide backbone with one core GlucNAc, and f the annotated MS/MS spectrum with the matched fragment ions in representative spectrum 9,487 of TR3