Fig. 3.

Quantification of up-regulated (2.00 ± 0.28) intact N-glycopeptide QVVENMTR_N2H3F0S0 from N-glycoprotein lysosomal alpha-glucosidase on N-glycosite N390 (P10253, LYAG_HUMAN) in MCF-7/ADR relative to MCF-7 cells. a–c the isotopic envelope fingerprinting maps of the precursor ions in the three technical replicates; d selective fragmentation and the graphical fragmentation map of N-glycan moiety with the peptide backbone, e fragmentation and the graphical fragmentation map of the peptide backbone with one core GlucNAc, and f the annotated MS/MS spectrum with the matched fragment ions in representative spectrum 13,312 of TR2