Fig. 5.

On N-glycosite N120 of serpin H1 (P50454), down-regulations of intact N-glycopeptides SLSNSTAR_N2HxF0S0 (x = 5–9) were gradually levelled off. a from N2H5F0S0 to N2H9F0S0, fold changes were 0.27 ± 0.03, 0.49 ± 0.08, 0.76 ± 0.08, 0.92 ± 0.08 and 1.09 ± 0.29, respectively; b the corresponding isotopic envelope fingerprinting maps of the precursor ions in this study; SLSNSTAR_N2H5F0S0 was identified as GPSMs and quantified with Top2 isotopic peaks of three technical replicates; SLSNSTAR_N2H9F0S0 was quantified at the monosaccharide composition level in two technical replicates