Table 1.
List of wheat endogenous gene(s) targeted using CRISPR in the literature
| Targeted gene(s) | Genome editing method | PAM type(s) | Repair result | Delivery | Efficiency | Reference |
|---|---|---|---|---|---|---|
| MLO | CRISPR/Cas9 | CCN | Indels | Particle bombardment | 5.6% | Wang et al. (2014) |
| GASR7, GW2, DEP1, NAC2, PIN1, LOX2 | CRISPR/Cas9 | CCN, NGG | Indels | Particle bombardment | 1.0–9.5% | Zhang et al. (2016) |
| EDR1 | CRISPR/Cas9 | CCN | Indels | Particle bombardment | N.A | Zhang et al. (2017) |
| gliadin | CRISPR/Cas9 | CCN | Indels | Particle bombardment | 62.3–75.1% | Sanchez-Leon et al. (2018) |
| PDS | CRISPR/Cas9 | CCN, NGG | Indels | Agrobacterium | 11–17% | Howells et al. (2018) |
| GW2, Lpx-1, MLO | CRISPR/Cas9 | CCN, NGG | Indels | Particle bombardment | 3–32% | Wang et al. (2018a) |
| CKX2-1, GLW7, GW2, GW8 | CRISPR/Cas9 | CCN, NGG | Indels | Agrobacterium | 10% | Zhang et al. (2019d) |
| Qsd1 | CRISPR/Cas9 | NGG | Indels | Agrobacterium | 37.5% | Abe et al. (2019) |
| DA1, DA2, NCED1, LPR2 | CRISPR/Cas9 | CCN, NGG | Indels | Agrobacterium | 20.8–54.2% | Zhang et al. (2019b) |
| GW7 | CRISPR/Cas9 | NGG | Indels | Particle bombardment | 1.1–8.3% | Wang et al. (2019) |
| Ms45 | CRISPR/Cas9 | NGG | Indels | Agrobacterium | N.A | Singh et al. (2018) |
| Ms1 | CRISPR/Cas9 | CCN, NGG | Indels | Agrobacterium | 5% | Okada et al. (2019) |
| NP1 | CRISPR/Cas9 | NGG | Indels | Particle bombardment | 14.0–54.2% | Li et al. (2020b) |
| Ms2 | CRISPR/Cas9 | CCN, NGG | Indels | Agrobacterium | 2.3–8.27% | Tang et al. (2020) |
| MTL, Waxy | CRISPR/Cas9 | CCN, NGG | Indels | Agrobacterium | 8.9–71.3% | Liu et al. (2020a) |
| Q | CRISPR/Cas9 | NGG | Indels | Agrobacterium | 44.0–45.6% | Liu et al. (2020b) |
| CENH3 | CRISPR/Cas9 | CCN, NGG | Indels, restored frameshift | Particle bombardment | N.A | Lv et al. (2020) |
| SBEIIa | CRISPR/Cas9 | CCN, NGG | Indels | Particle bombardment | 38.5–66.7% | Li et al. (2020c) |
| LOX2 | BE3 | NGG | C-to-T conversion | Particle bombardment | 1.25% | Zong et al. (2017) |
| ALS, ACCase | BE3 | CCN, NGG | C-to-T conversion | Particle bombardment | 2.5% | Zhang et al. (2019a) |
| ALS, MTL | A3A-PBE | NGG | C-to-T conversion | Particle bombardment | 16.7–22.5% | Zong et al. (2018) |
| DEP1, GW2 | ABE7.10 | NGG | A-to-G conversion | Particle bombardment | 0.4–1.1% | Li et al. (2018) |
| GW2, LOX2, MLO, GASR7, DME | Prime editing | NGG | Point mutations, targeted insertions and deletions | Particle bombardment | N.A | Lin et al. (2020) |
NA not available