Table 1.
List of targeted genes and traits modified via CRISPR/Cas technologies in Brassica
| Species | Targeted gene(s) | Editing methods | Repair results | Phenotype | Reference |
|---|---|---|---|---|---|
| Brassica oleracea var. capitata | BolC.GA4.a | CRISPR/Cas9 | Indels | Dwarf, and affected pod valve margin | Lawrenson et al. (2015) |
| Brassica oleracea var. capitata | BoPDS, BoSRK3, BoMS1 | CRISPR/Cas9, multiple gene editing | Indels | Albino phenotype, male sterility, self-compatibility | Ma et al. (2019a) |
| Brassica oleracea var. capitata | BoMYB28 | CRISPR/Cas9 | Indels | Reduced accumulation of methionine-derived glucosinolate | Neequaye et al. (2021) |
| Brassica oleracea var. capitata | BoCER1 | CRISPR/Cas9 | Indels | Reduced wax content, and brilliant green leaf | Cao et al. (2021) |
| Brassica oleracea var. capitata | BoPDS, BoFRI | CRISPR/Cas9, RNP | Indels | N.A | Murovec et al. (2018) |
| Brassica oleracea var. capitata | BoGI | CRISPR/Cas9, RNP | Indels | N.A | Park et al. (2019) |
| Brassica oleracea var. capitata | BoPDS | CRISPR/Cas9 | Indels | Albino phenotype | Ma et al. (2019b) |
| Brassica oleracea var. capitata | BoFAD2 | CRISPR/Cas9, double haploid inducer-mediated multiple gene editing | Indels | N.A | Li et al. (2021a) |
| Brassica oleracea var. alboglabra | BoaCRTISO | CRISPR/Cas9 | Indels | Yellow leaves and bolting stems | Li et al. (2020a) |
| Brassica rapa | BraPDS, BraFRI | CRISPR/Cas9, RNP | Indels | N.A | Murovec et al. (2018) |
| Brassica rapa | BraFLCs | CRISPR/Cas9, multiple gene editing | Indels | Early-flowering | Jeong et al. (2019) |
| Brassica rapa | BraHINS2 | CRISPR/Cas9 | Indels | Etiolated and senesced at the cotyledon-seedling stage | Su et al. (2021) |
| Brassica campestris | Bra003491, Bra007665, Bra014410 | CRISPR/Cas9, multiple gene editing | Indels | Methylation of pectin | Xiong et al. (2019) |
| Brassica carinata | BcFLA1 | CRISPR/Cas9 | Indels | N.A | Kirchner et al. (2017) |
| Brassica napus L | BnALC | CRISPR/Cas9 | Indels | Increased shatter resistance | Braatz et al. (2017) |
| Brassica napus L | BnMAX1 | CRISPR/Cas9, multiple gene editing | Indels | Decreased plant height, increased branch and silique numbers | Zheng et al. (2020) |
| Brassica napus L | BnCLV3 | CRISPR/Cas9 | Indels | Increased locule number of siliques | Yang et al. (2018) |
| Brassica napus L | BnFAD2 | CRISPR/Cas9, double haploid inducer-mediated multiple gene editing | Indels | Increased oleic acid content |
Okuzaki et al. (2018; Huang et al. (2020) |
| Brassica napus L | BnTT8 | CRISPR/Cas9 | Indels | Yellow-seeded traits | Zhai et al. (2020) |
| Brassica napus L | BnTT2 | CRISPR/Cas9 | Indels | Yellow-seeded traits | Xie et al. (2020) |
| Brassica napus L | BnWRKY70 | CRISPR/Cas9 | Indels | Enhanced resistance to Sclerotinia | Sun et al. (2018) |
| Brassica napus L | BnGTR2 | CRISPR/Cas9, multiple gene editing | Indels | Small seeds with high oil content and low glucosinolates content | Tan et al. (2022) |
| Brassica napus L | BnaA06.GTR2 | CRISPR/Cas9 | Indels | Low seed glucosinolates content | He et al. (2022) |
| Brassica napus L | BnRGA, BnFUL, BnDA1, BnDA2 | CRISPR/Cas9, multiple gene editing | Indels |
Longer stems N.A |
Yang et al. (2017) |
| Brassica napus L | BnIND | CRISPR/Cas9 | Indels | Enhanced pod shatter resistance | Zhai et al. (2019) |
| Brassica napus L | BnSPL3 | CRISPR/Cas9, multiple gene editing | Indels | Developmental delay phenotype | Li et al. (2018) |
| Brassica napus L | BnSDG8 | CRISPR/Cas9 | Indels | Early flowering | Jiang et al. (2018) |
| Brassica napus L | BnLMI1 | CRISPR/Cas9 | Indels | Unlobed leaves | Hu et al. (2018) |
| Brassica napus L | BnJAG | CRISPR/Cas9, multiple genome editing | Indels | Increased resistance to pod shattering | Zaman et al. (2019) |
| Brassica napus L | BnLPAT2, BnLPAT5 | CRISPR/Cas9, multiple genome editing | Indels | Increased the size of oil bodies and decreased the oil content | Zhang et al. (2019) |
| Brassica napus L | BnTFL1 | CRISPR/Cas9 | Indels | Early flowering phenotype and altered plant architecture | Sriboon et al. (2020) |
| Brassica napus L | BnA5.ZML1 | CRISPR/Cas9 | Indels | Reduced self-compatibility | Duan et al. (2020) |
| Brassica napus L | BnYCO | CRISPR/Cas9, multiple genome editing | Indels | Yellow cotyledon and chlorotic true leaves | Liu et al. (2021) |
| Brassica napus L | BnEOD3 | CRISPR/Cas9, multiple genome editing | Indels | Increased seed weight per plant | Khan et al. (2021) |
| Brassica napus L | BnA9.WRKY47 | CRISPR/Cas9 | Indels | Increased sensitivity to low boron | Feng et al. (2020) |
| Brassica napus L | BnZEP |
CRISPR/Cas9, multiple gene editing |
Indels | Orange-flowered rapeseed | Liu et al. (2020) |
| Brassica napus L | BnMS5 | CRISPR/Cas9, multiple gene editing | Indels | Male sterility | Xin et al. (2020) |
| Brassica napus L | BnD14 | CRISPR/Cas9, multiple gene editing | Indels | Prolific branching and dwarf | Stanic et al. (2021) |
| Brassica napus L | BnaA03.BP | CRISPR/Cas9, multiple gene editing | Indels | Semi-dwarf and compact architecture | Fan et al. (2021) |
| Brassica napus L | BnS6-SMI2 | CRISPR/Cas9 | Indels | Self-incompatibility | Dou et al. (2021) |
| Brassica napus L | BnaSVP | CRISPR/Cas9, multiple gene editing | Indels | Early-flowering phenotype | Ahmar et al. (2022) |
| Brassica napus L | BnALS | CBE | C to T | Herbicide-resistance | Wu et al. (2020) |
| Brassica napus L |
BnALS, BnRGA, BnIAA7 |
CBE | C to T |
Herbicide- resistance, semi-dwarf architecture |
Cheng et al. (2021) |
| Brassica oleracea var. botrytis |
BoALS BoCENH3 |
CBE | C to T |
Herbicide-resistance N. A |
Wang et al. (2022) |
| Brassica napus L | BnFT, BnPDS | ABE | A to G | N. A | Kang et al. (2018) |
Indels insertions and deletions, N.A. not available