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. Author manuscript; available in PMC: 2022 Oct 24.
Published in final edited form as: Cell Signal. 2018 Oct 19;53:234–245. doi: 10.1016/j.cellsig.2018.10.011

Fig. 5.

Fig. 5.

Dusp1, Dusp8 and Dusp16 regulate MAPK signaling in preadipocytes in response to TNFα. Preadipocytes were transfected with non-targeting control siRNA or siRNA specific for (A) Dusp1, (B) Dusp8, or (C) Dusp16 for 72 h prior to TNFα (100 pM) stimulation. Total RNA was harvested at the indicated times post-TNFα stimulation and qRT-PCR used to examine mRNA expression of each DUSP (left-panels). Data were normalized to 18S rRNA and changes in gene expression measured as fold differences relative to unstimulated control siRNA. Statistical differences were determined by ANOVA, with Tukey’s post-hoc analysis performed when the p value for the respective parameter was statistically significant (p < 0.05). Cell lysates were collected over time post-TNFα stimulation and immunoblotted for phospho-ERK, JNK, and p38 along with total JNK and GAPDH (right panels).