Skip to main content
. Author manuscript; available in PMC: 2022 Oct 24.
Published in final edited form as: Cell Signal. 2018 Oct 19;53:234–245. doi: 10.1016/j.cellsig.2018.10.011

Fig. 6.

Fig. 6.

Combination DUSP knockdown exacerbates MAPK signaling magnitude and duration in preadipocytes in response to TNFα. Preadipocytes were transfected for 72 h with non-targeting control siRNA or pooled siRNAs for Dusp1, Dusp8, and Dusp16 prior to TNFα (100 pM) stimulation. (A) Total RNA was harvested at the indicated times post-TNFα stimulation and qRT-PCR used to assess mRNA expression. Data were normalized to 18S rRNA and changes in gene expression measured as fold differences relative to unstimulated control siRNA. Significant differences were determined by ANOVA with Tukey’s post-hoc analysis performed when the p value for the respective parameter was statistically significant (p < 0.05). (B) Cell lysates were collected over time post-TNFα (100 pM) stimulation and immunoblotted for phospho-ERK, JNK, and p38 as well as α-Tubulin.