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. Author manuscript; available in PMC: 2022 Oct 24.
Published in final edited form as: Cell Signal. 2018 Oct 19;53:234–245. doi: 10.1016/j.cellsig.2018.10.011

Fig. 8.

Fig. 8.

JNK activation is essential for Dusp8 gene expression. A) Preadipocytes were pretreated (1 h) in the presence of inhibitors for ERK (U0126, 10 μM), JNK (SP600125, 20 μM), or p38 (SB203850, 10 μM) prior to TNFα (100 pM) stimulation. Total RNA was harvested 1 h post-TNFα. Dusp1, Dusp8, and Dusp16 mRNA expression was examined by qRT-PCR. Nomenclature assigned as: T (TNFα), T + E (TNFα + ERK), T + J (TNFα + JNK), T + P (TNFα + p38), or TEJP (TNFα +ERK + JNK + p38). (B) Preadipocytes were pretreated (1 h) with increasing doses of two independent JNK inhibitors (SP600125 or JNK inhibitor VIII) prior to 100 pM TNFα stimulation (1 h treatment). Total RNA was harvested and qRT-PCR used to assess Dusp8 mRNA expression. All data were expressed relative to unstimulated cells (NS) and normalized to 18S rRNA. Statistical significance was determined by ANOVA with Dunnett’s post-hoc analysis conducted to assess differences from TNFα when p < 0.05.