Table 2. Polymorphism information and amplification patterns of 10 RAPD primers during RAPD analysis in induced mutants of Lens culinaris Medik.
| S. No. | Primers | Nucleotide sequence (5ˈ-3ˈ) | No. of polymorphic bands | No. of monomorphic bands | Total no. of bands | Polymorphism (%) | G+C content (%) | Range (bp) |
|---|---|---|---|---|---|---|---|---|
| 1 | OPA-02 | TGCCGAGCTG | 6 | 3 | 9 | 66.66 | 70 | 200–1200 |
| 2 | OPA-05 | AGGGGTCTTG | 10 | 1 | 11 | 90.90 | 60 | 200–1600 |
| 3 | OPA-07 | GAAACGGGTG | 8 | 3 | 11 | 72.72 | 60 | 400–1200 |
| 4 | OPA-09 | GGGTAACGCC | 5 | 2 | 7 | 71.42 | 70 | 600–1500 |
| 5 | OPB-03 | CATCCCCFTG | 7 | 2 | 9 | 77.77 | 60 | 200–1600 |
| 6 | OPB-04 | GGACTGGAGT | 6 | 3 | 9 | 66.66 | 60 | 200–1800 |
| 7 | OPD-02 | GGACCCAACC | 5 | 3 | 8 | 62.50 | 70 | 200–1200 |
| 8 | OPC-05 | GATGACCGCC | 7 | 3 | 10 | 70.00 | 70 | 400–1800 |
| 9 | OPL-14 | GTGACAGGCT | 4 | 4 | 8 | 50.00 | 60 | 400–1600 |
| 10 | OPK-10 | GTGCAACGTG | 9 | 2 | 11 | 81.81 | 60 | 200–1200 |
| TOTAL | 67 | 26 | 93 | 710.44 | - | 200–1800 | ||
| Avg./primer | 6.7 | 2.6 | 9.3 | 71.04 |