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. 2022 Oct 24;17(10):e0276649. doi: 10.1371/journal.pone.0276649

Fig 2. A novel transgenic mouse model.

Fig 2

(A) In situ hybridization was used to detect APOL1 expression in the livers of mice expressing different alleles of APOL1 from a murine albumin promoter (Alb-G0, Alb-G1, and Alb-G2). Brown punctate staining represents detection of APOL1 RNA within hepatocytes. Scale bars are 50 μm. (B) APOL1 expressed in the liver circulates in plasma. Circulating human APOL1 was quantified in mouse plasma after the triple intervention by using a targeted LC-MS/MS assay. Data are displayed as mean ± SD. Statistical significance was determined using Student’s t-test. *P<0.05. (C) Compared with APOL1-G0, risk variant APOL1 resides on larger lipoprotein particles in mice. Asymmetric-flow field-flow fractionation of Alb/APOL1 mouse plasma demonstrated that APOL1-G1 is shifted to larger particles compared to APOL1-G0, similar to what is observed in human plasma. The effect was less substantial in Alb/APOL1-G2 mice.