FIG. 2.
Effect of dexamethasone pretreatment on subsequent stimulation of IL-6 production by CF and control B-cell lines. CF and control B cell lines were preincubated with dexamethasone (10−7 M) for 3 days. After three washes by centrifugation to remove dexamethasone, the B cells were stimulated with and without a cytokine-PMA cocktail (see Materials and Methods). Supernatant fluids were quantified for IL-6 by bioassay. Results are presented as the mean (± SD) percent inhibition of IL-6 production. The asterisks indicate no inhibition.