TABLE 1.
Oligonucleotides used to synthesize the cDNA fragments of SDAV genomic RNA
| cDNA fragment | Primer | Primer sequence | Nucleotide positions |
|---|---|---|---|
| f | 1b-fwd-nc | 5′-GCTGGTACGGCTGTTGTTAG-3′ | 1–10 |
| he-rev-nc | 5′-TCWGAACGACTGTCACC-3′ | 1095–1111 | |
| c | he-fwd-nc | 5′-GGTGACAGTCGTTCWGA-3′ | 1095–1111 |
| s1-rev-nc | 5′-GTAAGCAATAAYGTGG-3′ | 2484–2499 | |
| b | s1-fwd-nc | 5′-CYACRTTATTGCTTAMTGGG-3′ | 2484–2499 |
| hypvar-rev | 5′-AGAGTGACTCAGCCTGAACA-3′ | 3394–3413 | |
| a | 1stf | 5′-CCWCTMAAYTGGGAGCGTA-3′ | 3329–3347 |
| 1str | 5′-MGCWGAAACACGGCCRCTAT-3′ | 4320–4339 | |
| d | hypvar-fwd | 5′-GCAATTGTTCGGCTGATG-3′ | 3996–4013 |
| s2-rev-nc | 5′-TGTRCCAACKTCCTTGAG-3′ | 6164–6181 | |
| e | s2-fwd-nc | 5′-CTCAAGGAMGTTGGYACA-3′ | 6164–6181 |
| n1-rev-nc | 5′-TTGGGCTGAGTAGTTGCAGT-3′ | 8310–8329 | |
| g | nf1 | 5′-GCAGAAGCTCCTCTGGAAAC-3′ | 8191–8210 |
| ap1 | 5′-CCATCCTAATACGACCACTATAGGGC-3′ |
a
R = A + G; Y = C + T; M = A + C; K = G + T; W = A + T.