FIG. 2.
Identification and characterization of the membrane lipoprotein LppQ. (A) Immunoblots containing total antigens of M. mycoides subsp. mycoides SC strain Afadé were reacted with serum from a cow experimentally infected with the homologous strain (1) (lane a) or with monospecific polyclonal rabbit anti-LppQ′ antibodies (lane b). (B) Autoradiography of [14C]palmitate-labeled M. mycoides subsp. mycoides SC strain Afadé. Lane 1, total antigens; lane 2, Triton X-114 detergent phase; lane 3, aqueous phase. (C) Filter containing the same samples as in panel B reacted with anti-LppQ′ antibodies. The scale to the left of panel A is in kilodaltons.