TABLE 2.

Primers used in this study

Namea	Sequenceb	Tempc (°C)	Used
MMMLP481	cgccatatgCCATTAGTTGTTGTTTCATGTAAA	54	SDM, C
MMMLP482(r)	TCTTGTTTTTGCCACTCATTTTTTG	54	SDM
MMMLP483(f)	CAAAAAATGAGTGGCAAAAACAAGA	54	SDM
MMMLP484	ccggatccCCAATTTGATAAAACTTGATTAAAGT	54	SDM, C
P48B1f	aacgaattcAGTTTTATCAAATTGGAATACAGAAAAT	55	SDM, C
P48B1r	CATTTGCTGTTTTCCAGCTCGATAAATC	55	SDM
P48B2f	GTGATTTATCGAGCTGGAAAACAGCAAATG	55	SDM
P48B2r	CATTACTTACATTCCAACTCGAAATATCTT	55	SDM
P48B3f	AAGATATTTCGAGTTGGAATGTAAGTAATG	55	SDM
P48B3r	ATATTTTTTAGATTCCAATCTGAAAGTGAT	55	SDM
P48B4f	ATCACTTTCAGATTGGAATCTAAAAAATAT	55	SDM
P48B4r	CTTTTGATACATCCCAATCTAAAGACTTAT	55	SDM
P48B5f	ATAAGTCTTTAGATTGGGATGTATCAAAAG	55	SDM
P48B5r	TTTGAAACATTCCAATTAGTTATATTTTG	55	SDM
P48B6f	TCAAAATATAACTAATTGGAATGTTTCAAAT	55	SDM
P48B6r	ACATTATCAACTCTCCAATTTTTTATATCT	55	SDM
P48B7f	AGATATAAAAAATTGGAGAGTTGATAATGTA	55	SDM
P48B7r	ccggatccTACATTTTTTACATTCCAACTTGAAATATC	55	SDM, C
P48FBF	GTTTTATCAAATTGGAATACAGAAAATGTA	55	SDM
P48FAL	TACATTTTCTGTATTCCAATTTGATAAAAC	55	SDM
P48 EcoRI	gcagaattcCCATTAGTTGTTGTTTCATGTAAA	55	SDM, C
MMMSCO5-6	CAACCAAAGGCATCATATGA	47.5	P
MMMSCO5-7	CTCTGAGAGGGAATAATG	47.5	P

^af and r, forward and reverse mutagenesis primers. 

^bLowercase letters indicate the nucleotides that were added to the flanking primers in order to create restriction enzyme recognition sites (italics) for cloning. Substituted nucleotides for mutagenesis are underlined. 

^cAnnealing temperature for PCR amplification. 

^dSDM, site-directed mutagenesis; C, cloning; P, Identification of lppQ by PCR.