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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Cytometry A. 2022 Jan 31;101(5):448–457. doi: 10.1002/cyto.a.24536

Figure 1. Identification of poikilocytes in blood from SCD patients with imaging flow cytometry (IFC).

Figure 1.

Citrated whole blood from SCD patients was labeled with a FITC-conjugated antibody to CD235a, fixed with paraformaldehyde, and analyzed by IFC. (A) Enlarged images of a normally shaped RBC, a normally shaped RBC in side view, and a poikilocyte. R represents the radius of the cell; L and W are the length and width, respectively, of the bounding box around a cell. (B) Poikilocytes and normally shaped RBCs in side view (in the orange rectangle) have higher scores for elongatedness and lower scores for circularity than the normally shaped RBCs in frontal view. Elongatedness is the ratio of L over W of the bounding box of a cell. Circularity is a measure related to the radial variance of the cells. Biconcave cells have high circularity (low radial variance) whereas poikilocytes have low circularity (high radial variance). (C) Poikilocytes and RBCs in side view (in the orange rectangle) in panel (B) can be distinguished from each other based on cell area and modulation. See more details in METHODS.