Skip to main content
. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Cytometry A. 2022 Jan 31;101(5):448–457. doi: 10.1002/cyto.a.24536

Figure 4. Morphologies of CD71-positive RBCs in SCD blood.

Figure 4.

Citrated whole blood from SCD patients was labeled with antibodies to CD235a (FITC) and CD71 (APC), fixed with paraformaldehyde, and analyzed by IFC. (A) A scatter plot of RBCs with a gate for those positive for CD71. (B) CD71-positive RBCs with puckered shape were separated from those with biconcave shape using the plot of H contrast mean vs intensity object. (C) and (D) show representative brightfield and fluorescent images of the CD71-positive RBCs with puckered and biconcave morphologies, respectively. BF: brightfield, CD235a: glycophorin A, CD71: transferrin receptor, scale bar: 7 μm. (E) The level of CD71 was higher on the cells with puckered morphology than those with biconcave shape. MFI: Mean fluorescence intensity. Fourteen samples from 8 SCD patients were examined by IFC.