FIG. 4.

Recombinant M. tuberculosis antigens (arrows) purified by Ni-NTA affinity chromatography as observed on an SDS-PAGE gel (silver stained) (A) and on a Western blot of a duplicate gel which was probed with anti-RGS His antibody followed by detection with alkaline phosphatase-conjugated goat anti-mouse Ig and NBT-BCIP substrate (B). Some truncated products of the recombinant proteins were observed which were detected by the antibody. Lanes: M, Kaleidoscope prestained standards; 1, B.4; 2, B.6; 3, B.10; 4, B.M; 5, 38-kDa protein; 6, C17.