TABLE 2.
TB antigens genes which were cloned and expressed in pQE30a
| Antigen | Size of gene (kb) | Theoreticalb:
|
|
|---|---|---|---|
| Molecular mass (kDa) | pI | ||
| B.4 | 1.617 | 55.8 | 5.12 |
| B.6 | 1.560 | 55.0 | 5.03 |
| B.10 | 0.903 | 32.9 | 4.95 |
| B.M | 0.432 | 16.1 | 5.00 |
| C17 | 1.161 | 37.5 | 9.43 |
a
The resultant recombinant proteins will be approximately 1.4 to 1.5 kDa larger than the theoretical molecular mass shown, due to the histidine tag at the N terminus.
b
Obtained using the software “Compute pI/Mwt” from the ExPASy home page, Swiss Institute of Bioinformatics, Geneva, Switzerland.