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. 2022 Oct 10;12:1004014. doi: 10.3389/fonc.2022.1004014

Figure 3.

Figure 3

MBTPS1 is involved in proliferation of colon cancer-derived HT-29 and HCT-116 cells. (A) Inhibition of MBTPS1 enzymatic activity attenuates cell proliferation. Proliferation of HT-29 cells (green line) and HCT-116 (light blue line) treated with either vehicle or 10 μM PF-429242 for the entire duration of the experiment was measured using the XTT proliferation assay. Shown is an average ± SD of n=5 for each condition from four independent experiments. Dotted line represents proliferation of the untreated cells. (B, C) Rate of cell multiplication 96h after treatment of HT-29 (B) or HCT-116 (C) treated with either vehicle or 10 μM PF-429242. n=12 Student’s t-test ***p<0.0001. (D) Relative mRNA levels of MBTPS1 and its downstream targets 24 hours following treatment with 10 μM PF-429242 in HT-29 cells (D) and HCT-116 cells (E). The levels of each gene were compared between PF-429242-treated cells to vehicle. The experiment was conducted in n=3 biological repeats with three technical repeats in each experiment. Student’s t-test ***p<0.0001.