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. 2022 Oct 17;2022:2434938. doi: 10.1155/2022/2434938

Figure 5.

Figure 5

RGS2 was miR-452-5p's downstream target. (a) StarBase was utilized in the prediction of the miR-452-5p binding sites on RGS2. (b) Luciferase reporter experiment was conducted to assess the luciferase activities in the MDA-MB-2312 and HCC1937 cells that had a combined transfection of RGS2-WT or RGS2-MUT reporter plasmid plus either a miR-452-5p mimic or miR-NC. ∗∗P < 0.001 vs mimic-NC. (c) RT-qPCR was utilized for measuring RGS2 expression in BC as well as adjacent tissues. ∗∗P < 0.001. (d) RT-qPCR was utilized for the determination of RGS2 expression in normal MCF-10A cells and BC cells (MDA-MB-231 and HCC1937). ∗∗P < 0.001 vs MCF-10A. (e) The association between miR-452-5p and RGS2 levels in BC tissues was ascertained by Pearson's r. (f) Western blotting was utilized for measuring RGS2 protein expression in empty vector, mimic-NC, RGS2-OE (OE), mimic or OE+mimic transfected HCC1937, and MDA-MB-231 cells. ∗∗P < 0.001 vs empty vector. ^^P < 0.001 vs mimic-NC. ##P < 0.001 vs OE+mimic.