TABLE 3.
Bartonella genus-specific antigens detected by SDS-PAGE and Western blotting with antibodies against various Bartonella species
Protein size (kDa) | SDS- PAGE | Results of immunoblot with murine serum toa:
|
|||||
---|---|---|---|---|---|---|---|
bac | hen | qui | eli | gra | vin | ||
120 | + | ++ | + | ++ | ++ | + | + |
104 | + | + | ++ | ++ | ++ | ++ | + |
85 | + | + | ++ | + | + | + | − |
71 | + | + | + | − | +++ | − | − |
54 | ++++ | ++++ | ++++ | +++ | ++++ | ++++ | +++ |
47 | + | +++ | + | ++ | ++ | − | +++ |
40 | + | +++ | ++ | − | + | + | − |
33 | + | + | + | − | − | − | − |
30 | + | +++ | + | +++ | ++ | − | − |
19 | + | − | ++ | +++ | − | − | − |
Antigens were B. bacilliformis (bac), B. henselae (hen), B. quintana (qui), B. elizabethae (eli), B. grahamii (gra), and B. vinsonii (vin). −, no band evident; +, weak band; ++, moderate-intensity band; +++, intense band; ++++, very intense band.