Figure 4.

Microenvironmental landscaping and crosstalk influences epithelial stem cell phenotype

(A) Representative multiplex IHC images of immune, stromal, and matrix landscapes in mouse tumors selected from across the stem cell phenotypic axis. Scale bars, 100 μm.

(B) Variable proportion of different cell/matrix components in tumors from each genotype quantified from multiplex IHC images (n = 3 mice per genotype).

(C) Impact of media supplementation of IFN-γ (1 μL/mL) and TGFβ1 (0.5 μL/mL) on stem cell phenotype of wild-type and AKPT and KPN mouse tumor organoids. t test, p values as stated.

(D) Stem cell index applied to single-cell transcriptome data derived from organoids grown in Matrigel or collagen matrix (from Ramadan et al., 2021).