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. 2022 Oct 24;12:17804. doi: 10.1038/s41598-022-21758-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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A strategy for the construction of the mouse glycome atlas. (a) An image representation for the glycoblotting-based solid-phase glycan enrichment analysis for the construction of mouse tissue glycome atlas. (b) In the present study, sixteen frozen organs/tissues in addition to the serum and serum-derived exosomes were employed for the general protocol enabling one-pot rapid and efficient glycan enrichment and subsequent chemical manipulations on the polymer-solid beads. Pie charts represent ratio of the terminal sugar residues of each organ revealed by glycotyping analysis^17,18,37,49 of the present results. (c) General biosynthetic pathway of major N-glycans showing maturation from high mannose-type to hybrid-type and complex-type glycoforms in the posttranslational modification of mammalian proteins. This schematic representation does not involve the biosynthesis of minor glycoforms having GalNAc terminals, Lewis type antigenic structures, lactosaminoglycans (LacNAc repeats), sulfated sugar moieties, and so on. (d) The sugar compositions of N-glycans assigned based on the reported structures may often predict multiple candidates of the isomeric glycoforms found in the Expasy GlycoMod Tool (https://web.expasy.org/glycomod/ and https://glyconnect.expasy.org/browser/compositions/453).