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. 2022 Oct 24;12:17807. doi: 10.1038/s41598-022-21718-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characteristics of DSD case #7355: (a, b) normal male external genitalia; (c) histology of gonads: testes with degenerated spermatogenesis and rare sperms; (d) FISH with X (red) and Y (green) chromosome painting probes showing two cell lines: normal (38,XY) and monosomic (37,X); detection of Y-linked genes in blood leukocytes: (e) SRY: 1022 bp band detected by PCR, and (f) ZFY: 448 bp band (391 bp band represents the X-linked ZFX gene) detected by PCR–RFLP, L: 100 – 1000 bp ladder, lanes 1, 2: case #7355 (duplicated), M1, M2: control males; FM1, FM2: control females; 0: negative control.