TABLE 2.
Ratio statistics of genome cytosine methylation.
| Sample | Qx | Xht | RR | PQ | LL | QL |
|---|---|---|---|---|---|---|
| Methylated C sites | 667687248 | 687588186 | 594658757 | 671660061 | 650231100 | 582135310 |
| Un-methylated C sites | 11700079887 | 11846212052 | 10516549807 | 11757370201 | 11423526334 | 10126339596 |
| Total C sites | 12367767135 | 12533800238 | 11111208564 | 12429030262 | 12073757434 | 10708474906 |
| Methylation ratio of C site | 5.40% | 5.49% | 5.35% | 5.40% | 5.39% | 5.44% |
| Methylated C sites in CG sequence | 567751324 | 578483523 | 495273204 | 575718815 | 557911216 | 489209942 |
| Un-methylated C sites in CG sequence | 134356783 | 142470661 | 129100238 | 141419196 | 128842436 | 115048656 |
| Total C sites in CG sequence | 702108107 | 720954184 | 624373442 | 717138011 | 686753652 | 604258598 |
| Methylation ratio of C site in CG sequence | 80.86% | 80.24% | 79.32% | 80.28% | 81.24% | 80.96% |
| Methylated C sites in CHG sequence | 23148873 | 25521700 | 22715735 | 22043857 | 21004923 | 21299853 |
| Un-methylated C sites in CHG sequence | 2590704419 | 2644643902 | 2332157801 | 2625110648 | 2539644080 | 2260233698 |
| Total C sites in CHG sequence | 2613853292 | 2670165602 | 2354873536 | 2647154505 | 2560649003 | 2281533551 |
| Methylation ratio of C site in CHG sequence | 0.89% | 0.96% | 0.96% | 0.83% | 0.82% | 0.93% |
| Methylated C sites in CHH sequence | 76787051 | 83582963 | 76669818 | 73897389 | 71314961 | 71625515 |
| Un-methylated C sites in CHH sequence | 8975018685 | 9059097489 | 8055291768 | 8990840357 | 8755039818 | 7751057242 |
| Total C sites in CHH sequence | 9051805736 | 9142680452 | 8131961586 | 9064737746 | 8826354779 | 7822682757 |
| Methylation ratio of C site in CHH sequence | 0.85% | 0.91% | 0.94% | 0.82% | 0.81% | 0.92% |
Methylation ratio (%) = the number of methylation C sites in specific sequence environment/the total number of C sites * 100% 2 H represents A base or T base or C base