Skip to main content
. 2022 Oct 11;13:1010272. doi: 10.3389/fgene.2022.1010272

TABLE 1.

Primer codes and sequences of the forward (F) and reverse (R) primers used in the DREB analysis.

Codes Primer Primer sequence Fragment size (bp) Anneal temp. (°C)
DREB1-A F ATG​AAC​AGG​AAG​AAG​AAA​GTG​CGC 593 62
R TTC​TCA​AAT​CAT​TGC​TCA​CT TCTTTC
DREB1-A1 F CGG​AAC​CAC​TCC​CTC​CAT​CTC 1,107 58.8
R CGG​TTG​CCC​CAT​TAG​ACG​TCA
DREB1-A2 F CTG​GCA​CCT​CCA​TTG​CTG​AC 599 67.4
R AGT​ACA​TGA​ACT​CAA​CGC​ACA​GGA​CAA​C
DREB1-B F CCC​AAC​CCA​AGT​GAT​AAT​AAT​CT 816 58.8
R TTG​TGC​TCC​TCA​TGG​GTA​CTT
DREB1-B F ATG​ACC​AGG​AAG​AAG​AAA​GTG​CGC 585 60
R TCA​TTG​CTC​ACT​TCT​TTT​TTC​ACC​TTA​T
DREB1-D F ATG​AAC​AGG​AAG​AAG​AAA​GTG​CGC 455 52
R TCC​TTC​CCA​TCA​GAA​GGA​TGT​GAC
DREB1-D1 F TCG​TCC​CTC​TTC​TCG​CTC​CAT 1,190 69.6
R GCG​GTT​GCC​CCA​TTA​GAC​ATC​G
DREB1-D2 F CTG​GCA​CCT​CCA​TTG​CCG​AT 596 69.6
R AGT​ACA​TGA​ACT​CAA​CGC​ACA​GGA​CAA​C
DREB U F TCG​TCC​CTC​TTC​TCG​CTC​CAT​GG 493 66
DREB D R GGGCATGGCG CCGCATGG

Where (F), (R) indicates that the sequence of the forward and reverse primers, respectively.