TABLE 1.
Codes | Primer | Primer sequence | Fragment size (bp) | Anneal temp. (°C) |
---|---|---|---|---|
DREB1-A | F | ATGAACAGGAAGAAGAAAGTGCGC | 593 | 62 |
R | TTCTCAAATCATTGCTCACT TCTTTC | |||
DREB1-A1 | F | CGGAACCACTCCCTCCATCTC | 1,107 | 58.8 |
R | CGGTTGCCCCATTAGACGTCA | |||
DREB1-A2 | F | CTGGCACCTCCATTGCTGAC | 599 | 67.4 |
R | AGTACATGAACTCAACGCACAGGACAAC | |||
DREB1-B | F | CCCAACCCAAGTGATAATAATCT | 816 | 58.8 |
R | TTGTGCTCCTCATGGGTACTT | |||
DREB1-B | F | ATGACCAGGAAGAAGAAAGTGCGC | 585 | 60 |
R | TCATTGCTCACTTCTTTTTTCACCTTAT | |||
DREB1-D | F | ATGAACAGGAAGAAGAAAGTGCGC | 455 | 52 |
R | TCCTTCCCATCAGAAGGATGTGAC | |||
DREB1-D1 | F | TCGTCCCTCTTCTCGCTCCAT | 1,190 | 69.6 |
R | GCGGTTGCCCCATTAGACATCG | |||
DREB1-D2 | F | CTGGCACCTCCATTGCCGAT | 596 | 69.6 |
R | AGTACATGAACTCAACGCACAGGACAAC | |||
DREB U | F | TCGTCCCTCTTCTCGCTCCATGG | 493 | 66 |
DREB D | R | GGGCATGGCG CCGCATGG |
Where (F), (R) indicates that the sequence of the forward and reverse primers, respectively.