Table 3.
ddCts and percentage of the ace, esp, asa1, and efaA transcripts levels in penicillin-resistant, ampicillin-susceptible Enterococcus faecalis (PRASEF), and penicillin- and ampicillin-susceptible E. faecalis (PSASEF) exposed to sub-minimal inhibitory concentrations of gentamicin or ampicillin.
| Transcript | Antibiotics | ddCT PRASEF (Treated–Non-treated) |
ddCT PSASEF (Treated–Non-treated) |
||
|---|---|---|---|---|---|
| Mean ± SD (%) | P-value | Mean ± SD (%) | P-value | ||
| ace | Gentamicin | +0.16 ± 1.86 (−11.73%) | 0.874 | −0.12 ± 1.13 (+7.98%) | 0.842 |
| Ampicillin | +1.06 ± 1.33 (−108.49%) | 0.210 | +2.17 ± 1.01 (−350.02%) | 0.023 | |
| esp | Gentamicin | +0.05 ± 1.72 (−3.81%) | 0.948 | +0.43 ± 0.83 (−34.72%) | 0.376 |
| Ampicillin | −0.51 ± 1.37 (−42.41%) | 0.448 | −0.12 ± 2.48 (+7.98%) | 0.929 | |
| asa1 | Gentamicin | +0.37 ± 1.40 (−29.24%) | 0.636 | +1.60 ± 1.53 (−203.14%) | 0.128 |
| Ampicillin | +1.16 ± 1.40 (−123.46%) | 0.198 | +2.82 ± 0.90 (−606.16%) | 0.008 | |
| efaA | Gentamicin | −1.37 ± 1.84 (+61.31%) | 0.170 | −0.90 ± 0.75 (+46.41%) | 0.098 |
| Ampicillin | −0.37 ± 1.96 (+22.62%) | 0.695 | −0.96 ± 1.10 (+48.59%) | 0.177 | |
ddCt was determined considering the means of standardized Cts for PRASEF and PSASEF according to the absence or presence of antibiotics.
%, percentage of increase (+) or decrease (−) in the transcript level calculated from the ddCt.
SD, Standard Deviation.