REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Collagenase, Type 1	Worthington Biochemical Corporation	Cat#LS004196
Bovine Serum Albumin	Thermo Fisher Scientific	Cat#BP1600-1
Insulin solution	Sigma-Aldrich	Cat#I9278
Rosiglitazone	Sigma-Aldrich	Cat#557366
3,3′,5-Triiodo-L-thyronine (T3)	Sigma-Aldrich	Cat#T2877
HEPES	Sigma-Aldrich	Cat#H4034
D-(+)-Glucose	Sigma-Aldrich	Cat#G8270
D-(+)-Glucose solution	Sigma-Aldrich	Cat#G8769
Sodium pyruvate solution	Sigma-Aldrich	Cat#S8636
L-Glutamine solution	Sigma-Aldrich	Cat#G7513
Critical commercial assays
Aurum Total RNA Fatty and Fibrous Tissue Kit	Bio-Rad	Cat#7326830
iScript Reverse Transcription Supermix	Bio-Rad	Cat#1708841
Seahorse XFp Cell Mito Stress Test Kit	Agilent	Cat#103010-100
Seahorse XFp FluxPak	Agilent	Cat#103022-100
Seahorse XFe24 Islet Capture FluxPak	Agilent	Cat#103518-100
Pierce BCA Protein Assay Kit	Thermo Fisher Scientific	Cat#23227
Deposited data
RNA sequencing data	NCBI: Gene Expression Omnibus	NCBI GEO: GSE178720
CircaDB: Circadian gene expression profiles data base	Publicly available	http://circadb.hogeneschlab.org/mouse
Experimental models: Cell lines
Primary mouse brown adipocytes	mouse brown adipose tissue
Experimental models: Organisms/strains
Mouse: K15F/F:Klf15tm2Jain	Jain laboratory
Mouse: K15-BKO: Klf15tm2Jain x B6.FVB-Tg(Ucp1-cre)1Evdr/J	This manuscript
Mouse: Ucp1-cre: B6.FVB-Tg(Ucp1-cre)1Evdr/J	Jackson Laboratories
Oligonucleotides
See Table S1
Software and algorithms
Prism 9	GraphPad Prism
TopHat2	Johns Hopkins University Center for Computational Biology
Cufflinks	Cole Trapnell’s lab - University of Washington
Shinyheatmap	https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0176334
iDEP.96	https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-018-2486-6