REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Quantibrite Anti-Human HLA-DR PE/Monocyte PerCP-Cy™5.5 | BD Biosciences | Cat#340827; RRID: AB_400137 |
PE Phycoerythrin Fluorescence Quantitation Kit | BD Biosciences | Cat#340495; RRID: AB_2868736 |
CD20 Antibody, anti-human, FITC | Miltenyi Biotec | Cat#130-113-373; RRID: AB_2726142 |
PE Mouse Anti-Human CD14 | BD Biosciences | Cat#347497; RRID: AB_400312 |
Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscienceTM | Thermo Fisher Scientific | Cat#14-9161-73; RRID: AB_468582 |
PE Mouse Anti-Human CD14 | BD Biosciences | Cat#555398; RRID: AB_395799 |
PE-Cy™7 Mouse Anti-Human HLA-DR | BD Biosciences | Cat#560651; RRID: AB_1727528 |
Biological samples | ||
Blood sample from adult patient | Critical Care Unit of the Department of Anesthesiology, LariboisièreUniversity Hospital, Assistance Publique Hôpitaux deParis, France | Cochin Hospital Ethics Committee (# CCPPRB 2061) |
Blood sample from healthy blood donors | French national blood collection center (Etablissement Français du Sang, EFS) | N/A |
Chemicals, peptides, and recombinant proteins | ||
Recombinant Human Interferon Gamma (rh IFN-gamma) | ImmunoTools | Cat#11343537 |
Critical commercial assays | ||
iScript™ cDNA Synthesis Kit | Bio-Rad | Cat#1708891 |
Dual-Glo® Luciferase Assay System | Promega | E2940 |
SNP Genotyping Analysis Using TaqMan Assays | ThermoFisher Scientific | N/A |
TurboFect Transfection Reagent | ThermoFisher Scientific | Cat#R0531 |
Deposited data | ||
FHU Sepsis: personalized interventions | This paper | https://www.fhu-sepsis.uvsq.fr |
Experimental models: Cell lines | ||
U-937 | ATCC | CRL-15932 |
Raji | ATCC | CCL-86 |
Oligonucleotides | ||
SNP genotyping; CIITA-P3 SNP-712 Taqman probe: ATGGGAGTCAGTATTATTTAGCATC[A/G]CTTTGG CGGGTCACCCCAAACCATC |
This paper | N/A |
SNP genotyping; CIITA-P3 SNP-286 Taqman probe: GAAGTGAAATTAATTTCAGAGGTGT[A/G]GGGAG GGCTTAAGGGAGTGTGGTAA |
This paper | N/A |
QPCR CIITA isoforms; Promoter1 f: CATGGTGGCAGCTCAC | This paper | N/A |
QPCR CIITA isoforms; Promoter3 f: CCCAAGGCAGCTCACA | This paper | N/A |
QPCR CIITA isoforms; Promoter4 f: GAACAGCGGCAGCTCA | This paper | N/A |
QPCR CIITA isoforms; Exon2 r: GTAGCCACCTTCTAGGG | This paper | N/A |
HuRPLP03 f: AGGCTTTAGGTATCACCACTAA | This paper | N/A |
HuRPLP03 r: ACATCACTCAGGATTTCAATGG | This paper | N/A |
Recombinant DNA | ||
pGL3 Luciferase Reporter Vectors | Promega | E1761 |
pRL Renilla Luciferase Control Reporter Vectors TK | Promega | E2241 |
pRL Renilla Luciferase Control Reporter Vectors no promoter | Promega | E2271 |
Software and algorithms | ||
Arlequin ver 3.11 | Excoffier and Schneider, 2005 | http://cmpg.unibe.ch/software/arlequin3/ |
PLINK | Purcell et al., 2007 | https://zzz.bwh.harvard.edu/plink/ |
Stata | Stata Corp, 2016 | https://www.stata.com |
GraphPad Prism version 9.0 | GraphPad Software | www.graphpad.com |
FlowJo | BD Life Sciences | https://www.flowjo.com/solutions/flowjo |
Beacon Designer 7.0 | PREMIER Biosoft | http://www.premierbiosoft.com/molecular_beacons/ |
Other | ||
One-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol | Liu and Naismith, 2008 | https://pubmed.ncbi.nlm.nih.gov/19055817/ |