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. 2022 Oct 6;25(11):105291. doi: 10.1016/j.isci.2022.105291
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Quantibrite Anti-Human HLA-DR PE/Monocyte PerCP-Cy™5.5 BD Biosciences Cat#340827; RRID: AB_400137
PE Phycoerythrin Fluorescence Quantitation Kit BD Biosciences Cat#340495; RRID: AB_2868736
CD20 Antibody, anti-human, FITC Miltenyi Biotec Cat#130-113-373; RRID: AB_2726142
PE Mouse Anti-Human CD14 BD Biosciences Cat#347497; RRID: AB_400312
Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscienceTM Thermo Fisher Scientific Cat#14-9161-73; RRID: AB_468582
PE Mouse Anti-Human CD14 BD Biosciences Cat#555398; RRID: AB_395799
PE-Cy™7 Mouse Anti-Human HLA-DR BD Biosciences Cat#560651; RRID: AB_1727528

Biological samples

Blood sample from adult patient Critical Care Unit of the Department of Anesthesiology, LariboisièreUniversity Hospital, Assistance Publique Hôpitaux deParis, France Cochin Hospital Ethics Committee (# CCPPRB 2061)
Blood sample from healthy blood donors French national blood collection center (Etablissement Français du Sang, EFS) N/A

Chemicals, peptides, and recombinant proteins

Recombinant Human Interferon Gamma (rh IFN-gamma) ImmunoTools Cat#11343537

Critical commercial assays

iScript™ cDNA Synthesis Kit Bio-Rad Cat#1708891
Dual-Glo® Luciferase Assay System Promega E2940
SNP Genotyping Analysis Using TaqMan Assays ThermoFisher Scientific N/A
TurboFect Transfection Reagent ThermoFisher Scientific Cat#R0531

Deposited data

FHU Sepsis: personalized interventions This paper https://www.fhu-sepsis.uvsq.fr

Experimental models: Cell lines

U-937 ATCC CRL-15932
Raji ATCC CCL-86

Oligonucleotides

SNP genotyping; CIITA-P3 SNP-712 Taqman probe: ATGGGAGTCAGTATTATTTAGCATC[A/G]CTTTGG
CGGGTCACCCCAAACCATC
This paper N/A
SNP genotyping; CIITA-P3 SNP-286 Taqman probe: GAAGTGAAATTAATTTCAGAGGTGT[A/G]GGGAG
GGCTTAAGGGAGTGTGGTAA
This paper N/A
QPCR CIITA isoforms; Promoter1 f: CATGGTGGCAGCTCAC This paper N/A
QPCR CIITA isoforms; Promoter3 f: CCCAAGGCAGCTCACA This paper N/A
QPCR CIITA isoforms; Promoter4 f: GAACAGCGGCAGCTCA This paper N/A
QPCR CIITA isoforms; Exon2 r: GTAGCCACCTTCTAGGG This paper N/A
HuRPLP03 f: AGGCTTTAGGTATCACCACTAA This paper N/A
HuRPLP03 r: ACATCACTCAGGATTTCAATGG This paper N/A

Recombinant DNA

pGL3 Luciferase Reporter Vectors Promega E1761
pRL Renilla Luciferase Control Reporter Vectors TK Promega E2241
pRL Renilla Luciferase Control Reporter Vectors no promoter Promega E2271

Software and algorithms

Arlequin ver 3.11 Excoffier and Schneider, 2005 http://cmpg.unibe.ch/software/arlequin3/
PLINK Purcell et al., 2007 https://zzz.bwh.harvard.edu/plink/
Stata Stata Corp, 2016 https://www.stata.com
GraphPad Prism version 9.0 GraphPad Software www.graphpad.com
FlowJo BD Life Sciences https://www.flowjo.com/solutions/flowjo
Beacon Designer 7.0 PREMIER Biosoft http://www.premierbiosoft.com/molecular_beacons/

Other

One-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol Liu and Naismith, 2008 https://pubmed.ncbi.nlm.nih.gov/19055817/