Table 1. Competitive PEX Experiments (Percentage of Incorporation of Modified dNR)a.
| enzyme | Bst
LF |
Taq |
KOD
XL |
Pwo |
Vent(exo-) |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| dNTP/dNRTP | 1:1 | 1:10 | 1:1 | 1:10 | 1:1 | 1:10 | 1:1 | 1:10 | 1:1 | 1:10 |
| dCmTP | 76 (7) | 97 (1) | 71 (1) | 97 (1) | 66 (6) | 96 (2) | 78 (3) | 97 (3) | 73 (5) | 95 (3) |
| dChmTP | 11 (3) | 56 (2) | 17 (3) | 72 (2) | 47 (5) | 86 (1) | 53 (1) | 90 (2) | 50 (2) | 89 (2) |
| dCfTP | 42 (3) | 88 (2) | 62 (3) | 90 (4) | 77 (3) | 94 (3) | 76 (1) | 97 (1) | -b | -b |
| dUhmTP | 11 (2) | 59 (1) | 11 (3) | 54 (3) | 42 (3) | 91 (2) | 35 (4) | 83 (2) | 30 (1) | 83 (0) |
| dUfTP | 14 (2) | 63 (1) | 19 (1) | 67 (3) | 61 (3) | 86 (5) | 59 (5) | 88 (3) | 57 (5) | 88 (3) |
a
Standard deviations are in the parenteses. All experiments were performed in triplicate.
b
No PEX product was observed.