Table 4. Taq Kinetics.
| KM (μM) | kcat (s–1) | kcat/KM (s–1 μM–1) | ratea | |
|---|---|---|---|---|
| dCTP | 5.65 (0.35) | 0.54 (0.05) | 0.096 | 1.00 |
| dCmTP | 3.18 (0.19) | 0.49 (0.08) | 0.15 | 1.62 |
| dChmTP | 170 (38) | 0.81 (0.18) | 0.0047 | 0.05 |
| dCfTP | 3.73 (1.30) | 0.43 (0.10) | 0.11 | 1.19 |
| dTTP | 9.37 (0.65) | 0.45 (0.01) | 0.049 | 1.00 |
| dUhmTP | 27.3 (4.9) | 0.17 (0.04) | 0.0061 | 0.13 |
| dUfTP | 23.1 (3.1) | 0.45 (0.05) | 0.019 | 0.40 |
a
The rate is defined as (kcat/KM)modified/(kcat/KM)natural. All experiments were performed in triplicate, only dChmTP experiments were performed as duplicate.