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| KM (μM) | kcat (× 10–3 s–1) | kcat/KM (×10–3 s–1 μM–1) | ratea | |
|---|---|---|---|---|
| dCTP | 1.88 (0.84) | 5.62 (0.36) | 2.99 | 1.00 |
| dCmTP | 1.65 (0.45) | 6.34 (0.60) | 3.84 | 1.29 |
| dChmTP | 9.76 (2.50) | 5.51 (0.19) | 0.564 | 0.19 |
| dCfTP | 7.72 (3.25) | 5.53 (0.24) | 0.716 | 0.24 |
| dTTP | 10.9 (3.4) | 5.47 (0.05) | 0.501 | 1.00 |
| dUhmTP | 28.8 (4.9) | 4.16 (0.31) | 0.144 | 0.29 |
| dUfTP | 13.6 (1.6) | 3.22 (0.11) | 0.238 | 0.47 |
The rate is defined as (kcat/KM)modified/(kcat/KM)natural. All experiments were performed in triplicate.
