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| KM (μM) | kcat (× 10–4 s–1) | kcat/KM (× 10–4 s–1 μM–1) | ratea | |
|---|---|---|---|---|
| dCTP | 0.56 (0.15) | 8.18 (0.39) | 14.67 | 1.00 |
| dCmTP | 0.41 (0.02) | 4.08 (0.18) | 9.98 | 0.68 |
| dChmTP | 0.49 (0.03) | 4.37 (0.25) | 8.87 | 0.60 |
| dCfTP | 0.41 (0.11) | 4.86 (0.40) | 11.91 | 0.81 |
| dTTP | 0.54 (0.19) | 3.13 (0.20) | 5.75 | 1.00 |
| dUhmTP | 0.83 (0.25) | 2.84 (0.06) | 3.44 | 0.60 |
| dUfTP | 0.54 (0.07) | 3.73 (0.34) | 6.97 | 1.21 |
The rate is defined as (kcat/KM)modified/(kcat/KM)natural. All experiments were performed in triplicate.
