Table 3. AlphaScreen Signal Response for Biotinylated Peptides with Proteins Bearing Inactive Mutations^a.

^aPeptides were serially diluted 1:2. A heatmap display is used, with high percentage activity shown in red and low percentage activity shown in green. High percentage activity indicates binding of the peptide to the protein; reduced percentage activity indicates reduced binding. Percentage activity was calculated using the equation shown in the Supporting Information. Binding curves generated using GraphPad Prism are shown in the Supporting Information.