FIG. 3.

Cell-free coculture supernatants confer an antiviral state upon primary human astrocytes. Dilutions of supernatants from CD4⁺ lymphocyte-astrocyte cocultures were applied to fresh astrocyte cultures for 72 h and then infected with CMV (RC256). Freeze-thawed lysates of infected cells were assayed for β-galactosidase activity 72 h postinfection. The data, expressed as percents suppression compared to untreated infected astrocyte cultures (means ± standard errors), were obtained from independent experiments using CD4⁺ T cells from four different seropositive donors.