Fig. 1.

Increased CBS expression in metastatic ductal adenocarcinoma of the pancreas. (A). Molecular characteristics of pancreatic cancer cell lines isolated from PDAC metastases and primary tumors [[25], [26],52]. (B). CBS mRNA levels in PDAC and non-metastatic primary tumor cell lines as measured by RT-qPCR. (C). Western blot analyses of CBS protein expression levels in PDAC and non-metastatic primary tumor cell lines. (D). CBS activities in PDAC cell lysates as measured by an LC-MS/MS based method using the EZ:faast kit (for more details see methods). The data are shown as mean ± SEM of 2 independent experiments.