Fig. 10.

Differential sensitivity of hepatocytes and cardiomyocytes to excess RSS. The viability of (A) primary cardiomyocytes and (B) hepatocytes after treatment with variable Na₂S₄ concentrations in serum-free medium for 24 h was determined using an MTT assay. Data represent the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, compared with Na₂S₄ untreated sample (0 μM). (C) Representative fluorescence microscopy images and (D) quantitation of the average ratio of red/green fluorescence of primary cardiomyocytes and hepatocytes loaded with the JC-1 probe of mitochondrial membrane potential, then treated with variable Na₂S₄ concentrations for 45 min. Blue: Hoechst 33342 (nucleus). Red/green: JC-1. Scale bars: 50 μm. Data represent the mean ± SEM (n = 3). **p < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)