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. 2022 Oct 17;57:102514. doi: 10.1016/j.redox.2022.102514

Fig. 7.

Fig. 7

SLC7A11 is an CysSSH exporter in HepG2 cells. (A) Intracellular and (B) extracellular concentrations of CysSSH from WT, SLC7A11-KO, and SLC7A11-KOKI cells cultured in HBSS containing 100 μM CysSSCys. Cells and media were collected at the indicated time points and their CysSSH concentrations quantified using LC-ESI-MS/MS Data represent the mean ± SEM (n = 3). *p < 0.05, compared with WT. (C) Intracellular and (D) extracellular concentrations of CysSSH from WT, SLC7A11-KO, and SLC7A11-KOKI cells treated with 400 μM Na2S4 in DMEM for 1 h. After treatment, cells were washed three times and incubated with HBSS containing 100 μM CysSSCys, then cells and media were collected at the indicated time points and their CysSSH concentrations quantified using LC-ESI-MS/MS. Data represent the mean ± SE (n = 3). *p < 0.05, **p < 0.01, compared with WT.