Fig. 4.

miR-143-3p negatively regulates BCL2 and SLC7A11 expression. A TargetScan 7.2 and Starbase 3.0 were used to predict the potential targets of miR-143-3p. B KEGG enrichment of the 392 genes by Metascape server. C RT-qPCR was performed to detect the expression of miR-143-3p in AC16 cells transfected with miR-143-3p mimic or inhibitor. D, E RT-qPCR (D) and western blot (E) were performed to detect the levels of BCL2 and SLC7A11 in AC16 cells transfected with miR-143-3p mimic or inhibitor. F The putative miR-143-3p bound sequence of the wild type (wt) and mutant (mut) sequence of BCL2 or SLC7A11 from the Starbase 3.0 database. G The dual-luciferase assays were performed to verify the binding relationship between BCL2 or SLC7A11 and miR-143-3p. H RT-qPCR was performed to measure the levels of BCL2 and SLC7A11 in AC16 cells infected with SEMA5A-IT1-overexpressing plasmids and exposure to H/R damage. **p < 0.01, ***p < 0.001 compared with the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 compared with the SEMA5A-IT1_OE group