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. 2022 Oct 25;27:95. doi: 10.1186/s11658-022-00395-9

Table 1.

The sequences of primer used for RT-qPCR

Gene name Primer sequence
NONHSAT100333

F: 5′-GTTTCTTTCATCATTTCAACTCTGGGTA-3′

R: 5′-CTGCATCCCTGTCCTGTCACTTC-3′

NONHSAT137362

F: 5′-TCTATTTCTGTGGGATCAGTGGT-3′

R: 5′-AAATCAATGAATCCAAAAGAAGG-3′

NONHSAT035383

F: 5′-CACAAAGGCACATACTAGCAGAC-3′

R: 5′-GTGTACGTATGTCTATGTGAATG-3′

NONHSAT138804

F: 5′-ACATTCCATACACTTACGCAGAC-3′

R: 5′-GACAATCTATTTCTTTGGGGACA-3′

NONHSAT095978

F: 5′-TTGCATATCAGGGTAATAATGGC-3′

R: 5′-CCTAGAAGAAATCGGTAAATTCCTAAAC-3′

Tubulin

F: 5′-GGGGAGATGTATGAAGATGATGACGA-3′

R: 5′-TGGGAGCCCTAATGAGCTGGTGA-3′

GAPDH

F: 5′-CCTGTTCGACAGTCAGCCG-3′

R: 5′-GAGAACAGTGAGCGCCTAGT-3′

miR-143-3p

F: 5′-CTGGCGTTGAGATGAAGCAC-3′

R: 5′-CAGAGCAGGGTCCGAGGTA-3′

U6

F: 5′-CTCGCTTCGGCAGCACA-3′

R: 5′-AACGCTTCACGAATTTGCGT-3′

F forward, R reverse