Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 25;23:228. doi: 10.1186/s13059-022-02779-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Assessment of in vivo OT indels introduced with Mmp9-Cr1 gRNA. A Schematic illustrating OT candidate sites (OCSs) identification and their confirmation procedure. OCSs for Mmp9-Cr1 gRNA were identified by CRISPR-KRISPR and by Cas-OFFinder. The fragments containing OCSs selected were PCR amplified from founder and wildtype (WT) mice. Amplicon sequencing of these fragments were performed using a high-throughput sequencer. B Manhattan plot of 802 cleavage sites detected by CRISPR-KRISPR. The length of bars represents CRISPR-KRISPR read count. The Mmp9-Cr1 on-target site is indicated in red. Top 47 OCSs are indicated in blue. C The top 47 OCSs detected by CRISPR-KRISPR and three predicted OCSs by Cas-OFFinder are shown. D Sequence logo of cleavage sites detected by CRISPR-KRISPR. E Venn diagram showing the number of OCSs and overlap between those OCSs predicted by Cas-OFFinder and CRISPR-KRISPR