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. Author manuscript; available in PMC: 2023 Aug 19.
Published in final edited form as: Biofabrication. 2022 Aug 19;14(4):10.1088/1758-5090/ac8621. doi: 10.1088/1758-5090/ac8621

Figure 4.

Figure 4.

Characterization of 3D CFs-laden Gel-NVs hydrogels. (A) Schematic representation of the GAPDH mRNA levels downregulation process. Created with BioRender.com. (B) Live/dead images of CFs-laden Gel-NVs hydrogels on Day 1. (C) Live/dead assay showing the viability of CFs-laden Gel-NVs hydrogels on Day 1 (n=9). (D) PrestoBlue results showing the cell proliferation of CFs-laden Gel-NVs hydrogels for 7 days (n=3). (E) Images of CFs-laden Gel-NVs hydrogels loaded with miRNA DY547 (red) and immunostained with F-actin (green) and DAPI (blue). (F) RT-PCR results of CFs-laden Gel-NVs hydrogels loaded with miRNA GAPDH (n=3). All data are expressed as mean ± standard deviation. Significance is indicated as *(p < 0.05) and **(p < 0.01).