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. 2022 Oct 24;3(4):101840. doi: 10.1016/j.xpro.2022.101840

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Evaluation of CD4 T cell functional polarization

The initial analysis of live CD8^-CD19^-CD4⁺ cells is shown in Figure 3. The live CD8^-CD19^-CD4⁺ cell population is further subdivided into naïve CD4 T cells (CD45RA⁺), serving as a negative control to define chemokine receptor gating, antigen-experienced non-Tfh CD4 T cells (CD45RA^-CXCR5^-) or Tfh cells (CXCR5^hiPD-1^hi, gating shown in Figure 3).

(A and B) These 3 cell populations are further subdivided into (A) Th1 (CXCR3⁺) polarized cells and (B) Th2 (CXCR3^-CCR4⁺CCR6^-) or Th17 (CXCR3^-CCR4⁺CCR6⁺) polarized cells.