Fig 2. Detection limit of HCV RNA standards.

The limit of detection of one-step RT-RPA was measured using HCV RNA synthetic standards. Real-time fluorescence measured from reaction tubes is plotted over 17-minutes for experimental data of 1000, 100, 10, and 1 copy per reaction. All reactions with input copies of RNA greater than or equal to 10 copies per reaction amplified in 15-minutes or less, while replicates with 1 copy per reaction and no template controls (NTCs) did not amplify. The inset table lists data for all input RNA concentrations tested. All concentrations were run with 4 replicates.